Vol. 9(4), pp. 155-161, August 2021
https://doi.org/10.14662/arjasr2021235
Copyright 2021
Author(s) retain the copyright of this article
ISSN: 2360-7874
http://www.academicresearchjournals.org/ARJASR/Index.htm
 

Research paper

Molecular Identification of Penicillin Resistant Staphylococcus aureus Isolated from Bovine Mastitic Udder in Sokoto Metropolis

 

1Kasarawa A. B. *2Salau I. A. and 3Lawal S. M.

 

1Department of Science Laboratory Technology, Umaru Ali Shinkafi Polytechnic, Sokoto "Arial","sans-serif""> 

*2Department of Biological Sciences, Federal University Gusau, Zamfara, Nigeria "Arial","sans-serif""> 

3Department of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto, Nigeria *Corresponding address: Department of Biological Sciences, Federal University Gusau, Zamfara, Nigeria Tel: +234(0) 8053569551 Email: ibrahimasalau@gmail.com

 

Accepted 31 August 2021

Abstract

This study was carried out to identify Penicillin Resistance Staphylococcus aureus isolates isolated from milk of mastitis cattle in Sokoto metropolis using Antibiogram and PCR assay. Twenty (20) milk samples of mastitis cattle were collected from cattle herds in Sokoto metropolis and were analyzed using Biochemical tests, antibiogram and PCR assay. Samples were inoculated using streaking methods and cultures were incubated at 37oC for 24 hours. The results of the study revealed eighteen (90%) were positive for bacterial growth and there were no growth from two (10%) samples. The bacterial pathogens recovered from mastitis milk samples were Staphylococcus aureus 15 (75%) and 3(15%) were isolates of Streptococcus agalactiae. Antibiogram of the most prevalent Staphylococcus aureus showed that the isolates were sensitive to Gentamycin (GEN), Sorofloxacin (SP), Ciprofloxacin (CPX) and Pefloxacin (PEP)but resistant to Cefradine (CH).The PCR assay confirmed the isolates to be Penicillin resistant Staphylococcus aureus and the genomic DNA was confirmed to harbor the SPA genes. It is recommended that a control strategy should be developed to prevent and control the deleterious consequences of the pathogen on human health since the milk is consumed by the general populace in the Study area.

 

Key words: Antibiogram, Staphylococcus, Molecular, Bovine and Mastitic Udder

 

How to cite this article (APA Style): Kasarawa A. B., Salau I. A. and Lawal S. M. (2021). Molecular Identification of Penicillin Resistant Staphylococcus aureus Isolated from Bovine Mastitic Udder in Sokoto Metropolis. Acad. Res. J. Agri. Sci. Res. 9(4): 155-161